Ingrid Cabrera, Elisa Elizondo, Olga Esteban, José Luis Corchero, Marta Melgarejo, Daniel Pulido, Alba Córdoba, Evelyn Moreno, Ugutz Unzueta, Esther Vazquez, Ibane Abasolo, Simó Schwartz, Jr., Antonio Villaverde, Fernando Albericio, Miriam Royo, Maria F. García-Parajo, Nora Ventosa*, and Jaume Veciana*
Nano Lett., 2013, 13 (8), pp 3766–3774
The integration of therapeutic biomolecules, such as proteins and peptides, in nanovesicles is a widely used strategy to improve their stability and efficacy. However, the translation of these promising nanotherapeutics to clinical tests is still challenged by the complexity involved in the preparation of functional nanovesicles and their reproducibility, scalability, and cost production. Here we introduce a simple one-step methodology based on the use of CO2-expanded solvents to prepare multifunctional nanovesicle-bioactive conjugates. We demonstrate high vesicle-to-vesicle homogeneity in terms of size and lamellarity, batch-to-batch consistency, and reproducibility upon scaling-up. Importantly, the procedure is readily amenable to the integration/encapsulation of multiple components into the nanovesicles in a single step and yields sufficient quantities for clinical research. The simplicity, reproducibility, and scalability render this one-step fabrication process ideal for the rapid and low-cost translation of nanomedicine candidates from the bench to the clinic.