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New Cover in Chemical Reviews on ionic liquids

In an article published in Chemical Reviews, Pankaj Bharmoria and co-workers study ionic liquids, and their properties to work with proteins.

Oriol
Oriol
02 April 2024

Ionic liquids can show interesting properties when dissolving, extracting, stabilizing and purifying properties. The article is titled " Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment " and has made it to the Cover of this journal.

Abstract

Proteins are highly labile molecules, thus requiring the presence of appropriate solvents and excipients in their liquid milieu to keep their stability and biological activity. In this field, ionic liquids (ILs) have gained momentum in the past years, with a relevant number of works reporting their successful use to dissolve, stabilize, extract, and purify proteins. Different approaches in protein-IL systems have been reported, namely, proteins dissolved in (i) neat ILs, (ii) ILs as co-solvents, (iii) ILs as adjuvants, (iv) ILs as surfactants, (v) ILs as phase-forming components of aqueous biphasic systems, and (vi) IL-polymer-protein/peptide conjugates. Herein, we critically analyze the works published to date and provide a comprehensive understanding of the IL-protein interactions affecting the stability, conformational alteration, unfolding, misfolding, and refolding of proteins while providing directions for future studies in view of imminent applications. Overall, it has been found that the stability or purification of proteins by ILs is bispecific and depends on the structure of both the IL and the protein. The most promising IL-protein systems are identified, which is valuable when foreseeing market applications of ILs, e.g., in “protein packaging” and “detergent applications”. Future directions and other possibilities of IL-protein systems in light-harvesting and biotechnology/biomedical applications are discussed.

Reference

Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment

Pankaj Bharmori, Alesia A. Tietze, Dibyendu Mondal, Tejwant Singh Kang, Arvind Kumar, and Mara G Freire

Chemical Reviews. 2024, 124, 6, 3037–3084

DOI: 10.1021/acs.chemrev.3c00551

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